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Distinctive spectrometer providing high spectral resolution
over a large wavelength range
Angewandte Physik & Elektronik GmbH
sales@ape-berlin.de
www.ape-berlin.com
High resolution
over a large wavelength range
waveScan USB laser spectrometer
Wavelength ranges 0.2µm ... 6. 3 µm
(depending on version)
Fiber input (user exchangeable)
optionally available
MeetusatPhotonicsWest Booth410 MeetusatBiOS Booth8412
Highest signal level and
best spectral resolution (< 10 cm- 1)
due to 2 ps pulses
Ready to use source for multi-modal imaging:
- Coherent Raman scattering
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- SHG imaging
Tuning ranges:
700 ... 960 nm (Signal)
1120 ... 1960 nm (Idler)
1031 nm (fundamental)
picoEmerald TM S
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The most popular NGS attaches myri-
ad cut strands—to be analyzed on a lab-
on-a-chip setup—to a series of micron-sized spheres that are held in a 2D array
by a pattern of tiny wells on a glass surface. These are then amplified (cloned)
so that each strand becomes a tightly localized cluster of identical strands. They
are then sequentially exposed to a DNA
synthesizing agent (polymerase) togeth-
er with alternately applied fluorescently
labeled bases and remov-
able terminating agents.
All four base types are
identically labeled.
Initial mapping with a
660 nm laser is followed by
excitation with a 532 nm
laser together with imag-
ing using a low-noise cam-
era. Each dot in the image
flashes when it picks up a
fluorescent base, enabling
a computer to record the
sequence. Software then analyzes all the
random strands, with read lengths up to
thousands of bases, using sequence over-
laps to assemble the sequence of the orig-
inal uncut length of DNA.
One particularly interesting meth-
od with novel optics has been de-
veloped by Pacific Biosciences. This
system uses zero-
mode waveguides
(ZMWs), optical
waveguides whose diameter is much
smaller than the wavelength of light.
On a so-called single-molecule real
time (SMRT) chip, ZMWs are creat-
ed by patterning circular holes (~ 70
nm diameter) in an aluminum film ( 100
nm thickness) deposited on a clear silica
substrate. A single polymerase enzyme
FIGURE 3. Raw fluorescent image shows the massive number of ZMW sites that can be probed on a single
SMRT chip (left). The temporal trace from just one site shows how the flashes of fluorescence are translated
into a sequence (right). (Courtesy Pacific BioSciences)
